3LR1
The crystal structure of the tungstate ABC transporter from Geobacter sulfurreducens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.736, 70.386, 91.161 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.540 - 1.800 |
R-factor | 0.18516 |
Rwork | 0.181 |
R-free | 0.26527 |
Structure solution method | SAD |
RMSD bond length | 0.023 |
RMSD bond angle | 1.954 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.540 | 1.847 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.091 | 0.817 |
Number of reflections | 19739 | |
<I/σ(I)> | 23.83 | 1.88 |
Completeness [%] | 98.3 | 96.72 |
Redundancy | 9 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M ammonium sulfate, 0.1M bis-tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |