3LQQ
Structure of the CED-4 Apoptosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97623 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 181.640, 181.640, 203.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.664 - 3.534 |
R-factor | 0.2768 |
Rwork | 0.275 |
R-free | 0.31120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a5y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.437 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.664 | 3.680 |
High resolution limit [Å] | 3.534 | 3.530 |
Rmerge | 0.089 | 0.508 |
Number of reflections | 20874 | |
<I/σ(I)> | 14.6 | 1.78 |
Completeness [%] | 98.8 | 94.1 |
Redundancy | 3.9 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris-HCl, pH 8.5, 0.7M sodium potassium tartrate, 200mM Cymal-1 (Anatrace), VAPOR DIFFUSION, HANGING DROP, temperature 291K |