3LPT
HIV integrase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.000, 72.000, 65.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.550 - 2.000 |
R-factor | 0.23321 |
Rwork | 0.231 |
R-free | 0.27235 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.147 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.990 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 13564 | |
<I/σ(I)> | 5.8 | 1.9 |
Completeness [%] | 99.5 | 7.2 |
Redundancy | 4.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 10% (w/v) PEG 8000, 0.1M Na cacodylate pH 6.5, 0.1M (NH4)2SO4, 5mM DTT, VAPOR DIFFUSION, temperature 277K |