3LPM
Crystal structure of putative methyltransferase small domain protein from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.250, 76.277, 143.409 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.400 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.399 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.085 | 0.029 | 0.895 |
| Number of reflections | 30634 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 97.8 | 99.6 | 82.7 |
| Redundancy | 3.7 | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 10% PEG 8000,0.1 M CHESS, 0.2 M NaCl, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
