3LP8
Crystal structure of phosphoribosylamine-glycine ligase from Ehrlichia chaffeensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.850, 74.160, 107.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.470 - 2.150 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb deposition 2ys7 modified with CCP4 program CHAINSAW |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.364 |
| Number of reflections | 21857 | |
| <I/σ(I)> | 22.53 | 3.7 |
| Completeness [%] | 97.7 | 84.3 |
| Redundancy | 7.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | HR INDEX SCREEN F2: 100MM TRIS PH 8.5, 20% PEG 2000 MME, 200MM TRIMETHYLAMINE-N-OXIDE; EHCHA.00685.A AT 22.5MG/ML, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
