3LOY
Crystal structure of a Copper-containing benzylamine oxidase from Hansenula Polymorpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 288.510, 91.065, 151.098 |
| Unit cell angles | 90.00, 117.23, 90.00 |
Refinement procedure
| Resolution | 37.420 - 2.000 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.19079 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oov |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.057 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.089 | 0.319 |
| Number of reflections | 200161 | |
| <I/σ(I)> | 13.382 | 3 |
| Completeness [%] | 90.6 | 65.72 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.55M potassium sodium tartrate tetrahydrate in 0.10M phosphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
