3LOV

Crystal structure of Putative protoporphyrinogen oxidase (YP_001813199.1) from Exiguobacterium sp. 255-15 at 2.06 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97894,0.91837,0.97916
Spacegroup name	P 43 21 2
Unit cell lengths	74.542, 74.542, 212.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.270 - 2.060
R-factor	0.199
Rwork	0.197
R-free	0.24100
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.566
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.270	43.270	2.130
High resolution limit [Å]	2.060	4.420	2.060
Rmerge	0.126	0.044	1.040
Number of reflections	37840	4168	3374
<I/σ(I)>	12.9	36.8	1.8
Completeness [%]	99.4	99.5	95.1
Redundancy	7.08	6.45	6.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2000M sodium acetate, 30.0000% polyethylene glycol 4000, 0.1M TRIS pH 8.5, Additive: 0.003 M flavin adenine dinucleotide (FAD), 0.003 M nicotinamide adenine dinucleotide (NAD), NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K