3LOQ
The crystal structure of a universal stress protein from Archaeoglobus fulgidus DSM 4304
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.308, 89.169, 137.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.240 - 2.320 |
R-factor | 0.1809 |
Rwork | 0.179 |
R-free | 0.22480 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.243 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.300 | 2.370 |
High resolution limit [Å] | 2.320 | 2.330 |
Rmerge | 0.079 | 0.762 |
Number of reflections | 38893 | |
<I/σ(I)> | 24.7 | 2.1 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.1M Sodium Acetate trihydrate 3.0M NaCl, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |