3LOM

CRYSTAL STRUCTURE OF GERANYLTRANSFERASE FROM Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	93
Detector technology	CCD
Collection date	2010-01-23
Detector	ADSC QUANTUM 315
Spacegroup name	P 43 21 2
Unit cell lengths	94.775, 94.775, 148.345
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.22235
Rwork	0.221
R-free	0.26676
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.218
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		0.550
Number of reflections	30725
<I/σ(I)>	6	2.2
Completeness [%]	99.9	100
Redundancy	9.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	294	0.1M PHOSPHATE-CITRATE PH 4.2, 200MM LITHIUM SULFATE, 20% PEG1000, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K