3LO3
The crystal structure of a conserved functionally unknown protein from Colwellia psychrerythraea 34H.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912, 0.97937 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.684, 109.503, 126.509 |
| Unit cell angles | 66.02, 80.80, 79.42 |
Refinement procedure
| Resolution | 43.034 - 2.383 |
| R-factor | 0.1946 |
| Rwork | 0.190 |
| R-free | 0.27660 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.000 | 2.440 |
| High resolution limit [Å] | 2.383 | 2.390 |
| Rmerge | 0.093 | 0.717 |
| Number of reflections | 103654 | |
| <I/σ(I)> | 13.9 | 1.8 |
| Completeness [%] | 98.0 | 96.9 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1M CHES, 20% w/v PEG8000, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
