3LNT
Crystal structure of phosphoglyceromutase from Burkholderia Pseudomallei 1710B with bound malonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-23 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 |
| Unit cell lengths | 119.620, 119.620, 103.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.340 - 2.100 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.340 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.721 |
| Number of reflections | 49080 | |
| <I/σ(I)> | 18.88 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.4 M SODIUM MALONATE, PH 7.0. Crystal transferred to 2.4 M Malonate, pH 7.0 plus 25% (v/v) Ethylene Glycol for Cryoprotection, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
