3LNQ

Structure of Aristaless homeodomain in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	42.350, 42.350, 162.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.939 - 2.250
R-factor	0.22797
Rwork	0.227
R-free	0.25105
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a01
RMSD bond length	0.011
RMSD bond angle	1.659
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.067	0.024	0.695
Number of reflections	7641	98	551
<I/σ(I)>	23.32	43.1	3.6
Completeness [%]	99.7	83.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl, 0.2 M sodium acetate trihydrate, 26% PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K