3LNP
Crystal Structure of Amidohydrolase family Protein OLEI01672_1_465 from Oleispira antarctica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.902, 77.902, 143.754 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.246 - 2.100 |
R-factor | 0.151 |
Rwork | 0.149 |
R-free | 0.18400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.056 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.250 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 30246 | |
<I/σ(I)> | 9.6 | 4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.8 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 9 % PEG8000, 0.2 M Calcium Acetate, 0.1 M sodium cacodylate pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |