3LNO
Crystal Structure of Domain of Unknown Function DUF59 from Bacillus anthracis
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.458, 78.556, 174.116 |
Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
Resolution | 45.940 - 2.100 |
R-factor | 0.168 |
Rwork | 0.165 |
R-free | 0.21800 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.335 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.940 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 42911 | |
<I/σ(I)> | 12.5 | 3.5 |
Completeness [%] | 96.3 | 92.9 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1 M Acetate, 20% (v/v) 1,4-butanediol, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |