3LNN

Crystal structure of ZneB from Cupriavidus metallidurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	I 41 2 2
Unit cell lengths	137.111, 137.111, 202.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.959 - 2.796
R-factor	0.2413
Rwork	0.237
R-free	0.31350
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.738
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.959	2.900
High resolution limit [Å]	2.796	2.800
Rmerge	0.101
Number of reflections	20621
<I/σ(I)>	21	2.06
Completeness [%]	85.0	100
Redundancy	13.5	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	0.1M Na-Acetate pH 5.4 1.9M Na-formate 3% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	0.1M Na-Acetate pH 5.4 1.9M Na-formate 3% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K