3LNL
Crystal structure of Staphylococcus aureus protein SA1388
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-25 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | H 3 |
| Unit cell lengths | 132.547, 132.547, 125.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.140 - 2.000 |
| R-factor | 0.15117 |
| Rwork | 0.149 |
| R-free | 0.19053 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.546 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.140 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.216 |
| Number of reflections | 50416 | |
| <I/σ(I)> | 18.01 | 1.96 |
| Completeness [%] | 94.7 | 73.8 |
| Redundancy | 14.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 0.1 M BIS-TRIS PROPANE, PH 8.5, 0.2 M MGCL2, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
