3LNF
Crystal structure of E-cadherin EC12 K14EW2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97897 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.107, 47.383, 69.897 |
| Unit cell angles | 70.58, 88.13, 75.50 |
Refinement procedure
| Resolution | 28.635 - 2.500 |
| R-factor | 0.1872 |
| Rwork | 0.184 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.337 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.111 |
| Number of reflections | 17683 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 98.6 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2M ammonium sulfate, 0.1M Tris-Cl pH 8.5, 15% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
