3LNC
Crystal structure of guanylate kinase from Anaplasma phagocytophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-12 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.530, 76.580, 103.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.2104 |
| Rwork | 0.209 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2anb modified with CCP4 program CHAINSAW |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.067 | 0.485 |
| Number of reflections | 38137 | |
| <I/σ(I)> | 19.34 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 290 | EBS JCSG+ SCREEN, B6: 100MM PHOSPHATE/CITRATE, 40% ETHANOL, 5% PEG 1000. ANPHA.01463.A AT 52MG/ML, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
