3LN6
Crystal structure of a bifunctional glutathione synthetase from Streptococcus agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-23 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0720 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 143.020, 143.020, 211.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.077 | 0.132 |
Number of reflections | 24531 | 1765 |
<I/σ(I)> | 23.9 | 1.8 |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 8.4 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.6M (NH4)2SO4, 100mM Tris-HCl pH 8.5, 12% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.6M (NH4)2SO4, 100mM Tris-HCl pH 8.5, 12% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |