3LMW
Crystal structure of iota-carrageenase family GH82 from A. fortis in absence of chloride ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-20 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.960, 101.390, 95.910 |
| Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
| Resolution | 69.060 - 2.600 |
| R-factor | 0.18412 |
| Rwork | 0.180 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1H80 without domain A |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.610 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER ((CCP4)) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.060 | 2.530 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.067 | 0.380 |
| Number of reflections | 34408 | |
| <I/σ(I)> | 14.31 | 1.87 |
| Completeness [%] | 85.2 | 44 |
| Redundancy | 6.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
