3LMO

Crystal Structure of specialized acyl carrier protein (RPA2022) from Rhodopseudomonas palustris, Northeast Structural Genomics Consortium Target RpR324

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97852
Spacegroup name	P 32 2 1
Unit cell lengths	62.730, 62.730, 60.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.430 - 2.000
R-factor	0.217
Rwork	0.216
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.033	0.086
Number of reflections	16068
<I/σ(I)>	51.2	14.6
Completeness [%]	89.8	67.4
Redundancy	6.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch, under oil	4.2	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M sodium Citrate (pH 4.2), 12% PEG 20k, and 0.1M lithium sulfate, Microbatch, under oil, temperature 277K