3LML

Crystal Structure of the sheath tail protein Lin1278 from Listeria innocua, Northeast Structural Genomics Consortium Target LkR115

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	91.314, 153.718, 217.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 3.300
R-factor	0.23
Rwork	0.230
R-free	0.26000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.133	0.452
Number of reflections	88701
<I/σ(I)>	16.71	4.7
Completeness [%]	99.9	100
Redundancy	6.3	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch, under oil	8.5	291	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M Tris pH 8.5, 25% PEG 3350, and 0.2M NaCl, Microbatch, under oil, temperature 291K