3LMJ

Structure of human anti HIV 21c Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9762
Spacegroup name	P 65 2 2
Unit cell lengths	98.103, 98.103, 220.324
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.800 - 2.200
R-factor	0.19397
Rwork	0.193
R-free	0.21858
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.557
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.800
High resolution limit [Å]	2.200
Number of reflections	32467
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	70% 2-methyl-2,4-pentanediol, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K