3LLM
Crystal Structure Analysis of a RNA Helicase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98000, 0.98020, 0.96860 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 113.700, 113.700, 141.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 98.510 - 2.800 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23800 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.457 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.510 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.010 | |
Total number of observations | 42589 | |
Number of reflections | 26628 | |
<I/σ(I)> | 14.1 | 0.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.08 M Sodium cacodylate, 0.16 M Calcium acetate hydrate, 14.4 % PEG 8000, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |