3LLM
Crystal Structure Analysis of a RNA Helicase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98000, 0.98020, 0.96860 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 113.700, 113.700, 141.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.510 - 2.800 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.457 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.510 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.010 | |
| Total number of observations | 42589 | |
| Number of reflections | 26628 | |
| <I/σ(I)> | 14.1 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.08 M Sodium cacodylate, 0.16 M Calcium acetate hydrate, 14.4 % PEG 8000, 20 % glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
