3LLL
Crystal structure of mouse pacsin2 F-BAR domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.390, 106.400, 125.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.195 - 3.300 |
R-factor | 0.2577 |
Rwork | 0.256 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3haj (human PACSIN 2) |
RMSD bond length | 0.001 |
RMSD bond angle | 0.402 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.200 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 20356 | |
<I/σ(I)> | 3.9 | 1.1 |
Completeness [%] | 95.7 | 96.2 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 1:1 mixture of 20mg/mL mPACSIN2 and reservoir containing 0.1M HEPES/NaOH pH7.5, 1.62-1.66M (NH4)2SO4, 0.03M KBr, 4-4.5% PEG-MME 550, VAPOR DIFFUSION, SITTING DROP, temperature 300K |