3LLB
The crystal structure of the protein PA3983 with unknown function from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.034, 73.034, 30.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.640 - 1.800 |
| R-factor | 0.18383 |
| Rwork | 0.181 |
| R-free | 0.24337 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.901 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.640 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.442 |
| Number of reflections | 7457 | |
| <I/σ(I)> | 57.03 | 3.1 |
| Completeness [%] | 96.5 | 77.74 |
| Redundancy | 15.7 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 25% PEG3350, MgCl2 0.2M, Bis-Tris 0.1M, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
