3LKP
Crystal Structure of HLA B*3501 in complex with influenza NP418 epitope from 1972 strain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.120, 82.020, 110.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.840 - 1.800 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.840 | 1.900 | |
| High resolution limit [Å] | 1.800 | 10.000 | 1.800 |
| Rmerge | 0.082 | 0.028 | 0.685 |
| Number of reflections | 44107 | 261 | 6522 |
| <I/σ(I)> | 15.78 | 45.3 | 2.7 |
| Completeness [%] | 99.8 | 84.2 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 15-25% PEG 4000, 0.2M NH4Acetate, 0.1M Na-Citrate, pH 5.6, vapor diffusion, hanging drop, temperature 277K |
