3LKI
Crystal Structure of Fructokinase with bound ATP from Xylella fastidiosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.221, 92.651, 179.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.900 - 2.040 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.22200 |
| Structure solution method | MOLREP |
| Starting model (for MR) | 3ljs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.040 | 2.030 |
| Number of reflections | 75052 | |
| <I/σ(I)> | 5.8 | 5.1 |
| Completeness [%] | 99.1 | 92.6 |
| Redundancy | 14.1 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 292 | 50mM Potassium Phosphate dihydrogen, 10% 1,4 Butanediol, 15% PEG 8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
