3LKE
Crystal structure of enoyl-CoA hydratase from Bacillus halodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.917, 52.519, 104.891 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.059 |
| Number of reflections | 88196 |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 25% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
