3LKB
Crystal structure of a branched chain amino acid ABC transporter from Thermus thermophilus with bound valine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.708, 83.176, 122.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.740 - 2.400 |
| R-factor | 0.17713 |
| Rwork | 0.174 |
| R-free | 0.23611 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.500 |
| Number of reflections | 31204 | |
| <I/σ(I)> | 6.5 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes, 1.4M Sodium citrate, 6% 2-propanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
