3LK5
Crystal structure of putative Geranylgeranyl pyrophosphate synthase from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 125.434, 125.434, 52.067 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 1.900 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.19800 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.609 |
| Number of reflections | 37221 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.7 | 95.6 |
| Redundancy | 5.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 30% PEG4000, 0.2M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
