3LK4
Crystal structure of CapZ bound to the uncapping motif from CD2AP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2008-11-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.728, 142.341, 192.999 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 19.984 - 1.990 |
| R-factor | 0.2277 |
| Rwork | 0.225 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lk2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.054 | 0.418 |
| Number of reflections | 358158 | |
| <I/σ(I)> | 24.7 | 3.3 |
| Completeness [%] | 82.6 | 76.8 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% PEG 400, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
