3LJY
Crystal structure of putative adhesin (YP_001304413.1) from Parabacteroides distasonis ATCC 8503 at 2.41 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97925,0.97876 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 65.951, 55.330, 120.613 |
Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
Resolution | 29.761 - 2.410 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22500 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.513 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.761 | 29.770 | 2.470 |
High resolution limit [Å] | 2.410 | 10.780 | 2.410 |
Rmerge | 0.070 | 0.028 | 0.598 |
Total number of observations | 1016 | 4459 | |
Number of reflections | 32190 | ||
<I/σ(I)> | 10.8 | 29.3 | 1.6 |
Completeness [%] | 98.1 | 93 | 83.6 |
Redundancy | 2.9 | 2.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 22.0000% polyethylene glycol 6000, 1.0000M lithium chloride, 0.1M TRIS pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |