3LJW
Crystal Structure of the Second Bromodomain of Human Polybromo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 31 |
| Unit cell lengths | 78.964, 78.964, 50.651 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.340 - 1.501 |
| R-factor | 0.1735 |
| Rwork | 0.173 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g0j |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.455 | 1.510 |
| Number of reflections | 59763 | 6101 |
| <I/σ(I)> | 35.4 | 6.17 |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 35% PEG 4000, 10 mM Sodium Acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
