3LJQ
Crystal Structure of the Glycosylasparaginase T152C apo-precursor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Spacegroup name | P 1 |
Unit cell lengths | 46.061, 52.533, 61.933 |
Unit cell angles | 81.47, 90.21, 104.93 |
Refinement procedure
Resolution | 31.740 - 1.900 |
R-factor | 0.154 |
Rwork | 0.154 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9gac |
RMSD bond length | 0.013 |
RMSD bond angle | 1.620 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.740 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.029 | 0.059 |
Number of reflections | 42606 | |
<I/σ(I)> | 26.7 | 13.3 |
Completeness [%] | 97.2 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 277 | 15% PEG 3350, 100 mM HEPES pH 7.5, 0.1% sodium azide, VAPOR DIFFUSION, HANGING DROP, temperature 277K |