3LJI
CRYSTAL STRUCTURE OF putative geranyltranstransferase from Pseudomonas fluorescens Pf-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.639, 48.340, 75.693 |
| Unit cell angles | 90.00, 121.22, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.390 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.299 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.410 |
| High resolution limit [Å] | 1.390 | 3.770 | 1.390 |
| Rmerge | 0.050 | 0.037 | 0.489 |
| Number of reflections | 99504 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 96.4 | 94.9 | 79.8 |
| Redundancy | 3.2 | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 30% PEG MME, 150 mM potassium bromide, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
