3LJF
The X-ray structure of iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-26 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.476, 103.785, 90.251 |
Unit cell angles | 90.00, 103.81, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1isa |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.092 |
Number of reflections | 51958 |
Completeness [%] | 99.4 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | Crystals of protein were obtained using 2.3-2.5M ammonium sulphate, 10 mM FeCl3, 100 mM HEPES pH 7.0, 3% (v/v) isopropanol, VAPOR DIFFUSION, HANGING DROP |