3LJE
The X-ray structure of zebrafish RNase5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-27 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 126.489, 126.489, 126.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.810 - 1.800 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ljd |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.095 |
Number of reflections | 16327 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | 32% MPEG 2K, 0.2M ammonium sulfate, 0.1M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |