3LJD

The X-ray structure of zebrafish RNase1 from a new crystal form at pH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-16
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	41.339, 48.971, 109.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.380
R-factor	0.148
Rwork	0.148
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1agi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.380
Rmerge	0.049
Number of reflections	41877
Completeness [%]	89.7
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	Drops were prepared by mixing equal volumes of protein solution (15 mg/ml) and reservoir solution (2M ammonium sulphate and 0.1M sodium acetate), pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K