3LJB
Structural basis of oligomerisation in the MxA stalk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.147, 57.366, 57.491 |
| Unit cell angles | 65.82, 88.47, 74.87 |
Refinement procedure
| Resolution | 19.950 - 2.400 |
| R-factor | 0.19634 |
| Rwork | 0.193 |
| R-free | 0.25681 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Initial model derived from a low-resolution SeMet data set |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.152 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.103 | 0.473 |
| Number of reflections | 21393 | |
| <I/σ(I)> | 14.91 | 3.76 |
| Completeness [%] | 97.1 | 96.7 |
| Redundancy | 3.52 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 300 | 5% PEG3350, 100 mM MES (pH 6.8), 100 mM MgCl2 and 0.01 mM Hexammine cobalt (III) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
