3LJ5
Full Length Bacteriophage P22 Portal Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 408.949, 408.949, 260.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.484 - 7.497 |
R-factor | 0.193 |
Rwork | 0.187 |
R-free | 0.26330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.168 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 7.670 |
High resolution limit [Å] | 7.497 | 7.500 |
Number of reflections | 27188 | |
<I/σ(I)> | 12.7 | 2.2 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% tert-Butanol, 70mM sodium chloride, 2.5% PEG 400, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |