3LIO
X-ray structure of the iron superoxide dismutase from pseudoalteromonas haloplanktis (crystal form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.866, 103.714, 50.242 |
| Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
| Resolution | 14.850 - 1.500 |
| Rwork | 0.185 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isa |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.060 |
| Number of reflections | 66818 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.8M ammonium sulphate, 1.0M sodium chloride, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
