3LII
Recombinant human acetylcholinesterase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Collection date | 2000-11-15 |
Wavelength(s) | 0.93 |
Spacegroup name | P 61 |
Unit cell lengths | 210.900, 210.900, 115.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.050 - 3.200 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.21966 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b41 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.368 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.100 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 47979 | |
<I/σ(I)> | 15.1 | 3.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 1.4M lithium sulfate, 0.1M HEPES, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |