3LIF
Crystal Structure of the extracellular domain of the putative histidine kinase rpHK1S-Z16
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.97918, 0.97935, 0.96788 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 184.780, 184.780, 184.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.390 - 2.700 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.25600 |
Structure solution method | MAD |
RMSD bond length | 0.021 |
RMSD bond angle | 2.417 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.065 | 0.035 | 0.352 |
Number of reflections | 56039 | ||
<I/σ(I)> | 12.4 | ||
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 12.9 | 12.7 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 293 | 0.2M NH4Ac, 0.1M citrate pH5.6, 30% MPD, vapor diffusion, temperature 293K |