3LID
Crystal Structure of the extracellular domain of the putative histidine kinase vpHK1S-Z8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 0.97924 0.97929 0.96784 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.102, 79.444, 123.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.807 - 1.760 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22200 |
Structure solution method | MAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.117 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 3.790 | 1.760 |
Rmerge | 0.054 | 0.046 | 0.256 |
Number of reflections | 72469 | ||
<I/σ(I)> | 18.7 | ||
Completeness [%] | 99.0 | 96.1 | 95.7 |
Redundancy | 7.2 | 11.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 293 | 5% PEG8K, 0.2M NaCl, 0.1M Na/K Phosphate pH6.2, vapor diffusion, temperature 293K |
1 | VAPOR DIFFUSION | 6.2 | 293 | 5% PEG8K, 0.2M NaCl, 0.1M Na/K Phosphate pH6.2, vapor diffusion, temperature 293K |