3LIC
Crystal Structure of the extracellular domain of the putative histidine kinase soHK1S-Z6
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97927, 0.97937, 0.96789 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.069, 133.069, 32.369 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.410 - 2.300 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.068 | 0.047 | 0.225 |
| Number of reflections | 28506 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6.9 | 7.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 293 | 5% PEG3350, 0.5M NH4SO4, 0.1M Na Citrate pH 5.8, vapor diffusion, temperature 293K |
