3LI9
Crystal Structure of the extracellular domain of the putative histidine kinase mmHK1S-Z2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-02 |
| Wavelength(s) | 0.97903, 0.97936, 0.97174 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.283, 88.495, 99.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.912 - 1.700 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.079 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.051 | 0.032 | 0.335 |
| Number of reflections | 62673 | ||
| <I/σ(I)> | 16.5 | ||
| Completeness [%] | 98.9 | 96.8 | 93.2 |
| Redundancy | 4.7 | 4.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 15% PEG3350, 0.21M NH4SO4, 0.1MBistris, pH 5.6, vapor diffusion, temperature 293K |
