3LHN

Crystal structure of putative lipoprotein (NP_718719.1) from Shewanella oneidensis at 1.42 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97932,0.97882
Spacegroup name	P 61 2 2
Unit cell lengths	50.185, 50.185, 300.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.421 - 1.420
R-factor	0.173
Rwork	0.172
R-free	0.18100
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.772
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.421	28.421	1.470
High resolution limit [Å]	1.420	3.060	1.420
Rmerge	0.035	0.019	0.492
Number of reflections	43813	7996	7469
<I/σ(I)>	18.52	53.5	1.7
Completeness [%]	98.7	98.8	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	1.6000M ammonium sulfate, 0.1M citric acid pH 5.0, 0.001 M copper (II) chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K