3LHH
The crystal structure of CBS domain protein from Shewanella oneidensis MR-1.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.605, 55.257, 78.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.578 - 2.100 |
| R-factor | 0.2031 |
| Rwork | 0.200 |
| R-free | 0.26530 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.600 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.101 | 0.797 |
| Number of reflections | 10353 | |
| <I/σ(I)> | 28.1 | 1.52 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 8.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M Tri-Na-Citrate, 2% MPD, 25% PEG3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
