3LHF

The Crystal Structure of a Serine Recombinase from Sulfolobus solfataricus to 2.3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-20
Detector	ADSC QUANTUM 315
Spacegroup name	P 41
Unit cell lengths	83.363, 83.363, 83.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.460 - 2.300
R-factor	0.181
Rwork	0.178
R-free	0.22600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.071	0.538
Number of reflections	49014
<I/σ(I)>	10.4
Completeness [%]	98.7	99.7
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	277	25.5% PEG 4000, 15% glycerol, 0.085M Sodium acetate pH 4.6, 0.17M Ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K